lipofectamine® ltx reagent with plustm reagent Search Results


imageproplus software  (Cybergenetics Corporation)
90
Cybergenetics Corporation imageproplus software
Imageproplus Software, supplied by Cybergenetics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/gel+pro+analyzer+software/pm14523052-75-170-200
Average 90 stars, based on 1 article reviews
imageproplus software - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
steadylite plustm promega  (Promega)
90
Promega steadylite plustm promega
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Steadylite Plustm Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/steadylite+plustm+promega/pmc03669402-76-0-2
Average 90 stars, based on 1 article reviews
steadylite plustm promega - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
uvinul a plustm  (BASF)
90
BASF uvinul a plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Uvinul A Plustm, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/uvinul+a+plus/us08586011-322-15-19
Average 90 stars, based on 1 article reviews
uvinul a plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
fiveeasy plustm  (METTLER TOLEDO)
90
METTLER TOLEDO fiveeasy plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Fiveeasy Plustm, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/ph+meter+fe20/pmc11861095-62-14-16
Average 90 stars, based on 1 article reviews
fiveeasy plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
genjet plustm  (SignaGen)
90
SignaGen genjet plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Genjet Plustm, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/genjet+plustm/pm32297364-36-30-35
Average 90 stars, based on 1 article reviews
genjet plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
insulated isothermal pcr (ii pcr) iq plustm  (Genereach corp)
90
Genereach corp insulated isothermal pcr (ii pcr) iq plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Insulated Isothermal Pcr (Ii Pcr) Iq Plustm, supplied by Genereach corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/insulated+isothermal+pcr++ii+pcr++iq+plustm/10__1080_slash_09712119__2017__1285771-63-12-20
Average 90 stars, based on 1 article reviews
insulated isothermal pcr (ii pcr) iq plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rnasintm plus  (Promega)
90
Promega rnasintm plus
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Rnasintm Plus, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/rnasintm+plus/us08241860-195-24-26
Average 90 stars, based on 1 article reviews
rnasintm plus - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
facstar plustm  (Becton Dickinson)
90
Becton Dickinson facstar plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Facstar Plustm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/facstar+plustm/pmc02195719-54-20-22
Average 90 stars, based on 1 article reviews
facstar plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
total plustm pak  (alcon inc)
90
alcon inc total plustm pak
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Total Plustm Pak, supplied by alcon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/total+plustm+pak/us07244240-35-8-12
Average 90 stars, based on 1 article reviews
total plustm pak - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
natalasetm  (Novozymes limited)
90
Novozymes limited natalasetm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Natalasetm, supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/natalasetm/us09988616-1856-8-13
Average 90 stars, based on 1 article reviews
natalasetm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
exolution plustm  (Exosome Diagnostics)
86
Exosome Diagnostics exolution plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Exolution Plustm, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/exolution+plustm/us12378608-568-14-26
Average 86 stars, based on 1 article reviews
exolution plustm - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
newcast plustm  (Visiopharm AS)
90
Visiopharm AS newcast plustm
A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. <t>Steadylite</t> Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.
Newcast Plustm, supplied by Visiopharm AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine%C2%AE+ltx+reagent+with+plustm+reagent/newcast+plustm/pm28314804-418-27-30
Average 90 stars, based on 1 article reviews
newcast plustm - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. Steadylite Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.

Journal: PLoS ONE

Article Title: A Novel High Throughput Invasion Screen Identifies Host Actin Regulators Required for Efficient Cell Entry by Toxoplasma gondii

doi: 10.1371/journal.pone.0064693

Figure Lengend Snippet: A. HeLa-GFP cells were transfected with non-targeting (NT) siRNA or siRNA targeting GFP by reverse transfection and examined by immunofluorescence 48 h and 72 h post-transfection. B. HeLa-GFP cells were mock-transfected or transfected with non-targeting siRNA or siRNA against GFP and analyzed by immunoblotting 72 h post-tranfection using anti-GFP or anti-actin antibodies. C. HeLa cells were reverse-transfected with either non-targeting siRNA (lysed cells, NTsiRNA or tachyplegin) or PLK1 in 384-wells. 48 h post-transfection, a set of HeLa cells transfected with NT siRNA were treated with passive lysis buffer to lyse cells. 72 h post-transfection, freshly harvested U-Luc parasites untreated or treated with tachyplegin (chemical inhibitor of invasion) were added onto host cells and incubated at 37°C for 3 h. Steadylite Plus™ was added to the plate and luciferase activity was measured using PHERstar system. n = 3 independent experiments each with triplicate samples. Error bars, SEM. D. Host cell viability was assessed by adding CellTiter-Fluor™ and the values obtained with cells transfected with NT siRNA only were set at 100%. n = 3 independent experiments each with triplicate samples. Error bars, SEM.

Article Snippet: Steadylite PlusTM (Promega) was dispensed onto the plates (10 μl/well), incubated at RT for 10 min and luminescence values reflecting parasite invasion were measured with the PHERstar plate reader.

Techniques: Transfection, Immunofluorescence, Western Blot, Lysis, Incubation, Luciferase, Activity Assay

A. Eighteen target genes represented in the library were silenced by siRNA transfection of HeLa cells in 96 well plates. The percent decrease in mRNA levels of target genes was determined using qRT-PCR 72 h post-transfection. n = 2 independent experiments, each with triplicate samples. Error bars, SEM. B. Schematic flowchart showing the siRNA screening strategy for identification of host factors playing a role in Toxoplasma invasion. The strategy consisted of three main steps of transfection, invasion and measurement. Host cell viability was assessed using CellTiter-Fluor™, which measures protease conversion of a quenched membrane permeable peptide substrate (glycine-phenylalanine-7-Amino-4-trifluormethylcoumarin). Parasite invasion was assessed using the luciferin-based reagent Steadylite Plus™, which measures the inducible luciferase activity of U-luc parasites.

Journal: PLoS ONE

Article Title: A Novel High Throughput Invasion Screen Identifies Host Actin Regulators Required for Efficient Cell Entry by Toxoplasma gondii

doi: 10.1371/journal.pone.0064693

Figure Lengend Snippet: A. Eighteen target genes represented in the library were silenced by siRNA transfection of HeLa cells in 96 well plates. The percent decrease in mRNA levels of target genes was determined using qRT-PCR 72 h post-transfection. n = 2 independent experiments, each with triplicate samples. Error bars, SEM. B. Schematic flowchart showing the siRNA screening strategy for identification of host factors playing a role in Toxoplasma invasion. The strategy consisted of three main steps of transfection, invasion and measurement. Host cell viability was assessed using CellTiter-Fluor™, which measures protease conversion of a quenched membrane permeable peptide substrate (glycine-phenylalanine-7-Amino-4-trifluormethylcoumarin). Parasite invasion was assessed using the luciferin-based reagent Steadylite Plus™, which measures the inducible luciferase activity of U-luc parasites.

Article Snippet: Steadylite PlusTM (Promega) was dispensed onto the plates (10 μl/well), incubated at RT for 10 min and luminescence values reflecting parasite invasion were measured with the PHERstar plate reader.

Techniques: Transfection, Quantitative RT-PCR, Membrane, Luciferase, Activity Assay